![]() Alexa Fluor 555-labeled spores were injected (10 7 spores) IP in C57BL/6 or CD14 −/− mice. ( C) Internalization of fluorescent spores by peritoneal macrophages. Proportions of cells bearing spores in the wt ( A) and CD14 −/− ( B) populations were determined by flow cytometry. THP-1 cells stably transfected with either CD14 or vector alone were exposed to wt, Δ rmlD or Δ bclA Alexa 488-spores. anthracis spores by phagocytic cells is a dynamic process and involves multiple receptors and signaling pathways.īinding to CD14 correlates with spore internalization. Our results show that the binding and uptake of B. anthracis spore challenge of CD14(-/-) mice, interference with the CD14-mediated signaling pathways results in increased mortality. As observed with mice lacking Mac-1, CD14(-/-) mice are also more resistant than wild-type mice to infection by B. In this process, CD14 induces signals involving TLR2 and PI3k that promote inside-out activation of Mac-1, thereby enhancing spore internalization by macrophages. We show that CD14 binds to rhamnose residues of BclA and acts as a coreceptor for spore binding by Mac-1. Recognition of BclA by the integrin Mac-1 promotes spore uptake by professional phagocytes, resulting in the carriage of spores to sites of spore germination and bacterial growth in distant lymphoid organs. anthracis spores contains an external hair-like nap formed by the glycoprotein BclA. open tginstall.Anthrax, a potentially lethal disease of animals and humans, is caused by the Gram-positive spore-forming bacterium Bacillus anthracis.UPDATE: TUAW is reporting a reader named Clancy found a fix for running on GMA 950 Macs. After all, waiting for platform equivalency is as much a part of Mac gaming as the playing. The web page states that you will be able to buy Creature Creator for the Mac soon, but I might just wait for the full game to be released. You can still create creatures and share them with the central database now-a big part of game play in Spore-but creatures created on Windows PCs with the paid version of Creature Creator will be better. You need to pay $10 to get the fully functional game, which will be included as part of Spore when it is released later this year. Unfortunately, the demo only allows you to choose a few parts when creating your creatures. Once I fixed that and cranked up the graphics, Spore looked and ran great on a 2.0 GHz Core Duo iMac, at least as much of it as I was able to run. The Mac version started up at a default resolution of 1024 x 768, which stretched like crap on an iMac at 1680 x 1050. Running at 1680 x 1050 does require full-screen mode to access the settings menu, though. As Open Forum member Muriac pointed out, Command+Return toggles between full screen and whatever resolution you have it set at. My first disappointment was that Creature Creator only runs in full-screen mode if you don't read the directions. And not for you Mac mini owners, and anyone who purchased a MacBook before late last year, as "this game will not run on the GMA 950 class of integrated video cards." For those left, you can run the Spore demo, but with a few restrictions that will make you feel like the second-class gaming citizen that you, as a Mac user, are. At least 260MB of hard drive space for installation, plus additional space for created creatures.ATI X1600 or NVidia 7300 GT with 128 MB of Video RAM, or Intel Integrated GMA X3100. ![]() The bad news is the system requirements will be keeping more than a few Mac users from playing iGod. Before that, at Macworld Expo 2008 the Creature Creator was up and running, and now it's available as a demo to download. A mobile component of the game was previewed for the iPhone as part of the Apple Event in March. Spore has also been in the news for the Mac this year. ![]() Broken into five distinct phases of game play: tide pool, creature, tribal, civilization, space, you play god-though it's more the watchmaker than the Zeus-type throwing lightning bolts and taking the shape of animals to have sex. ![]() Will Wright of Sims fame has created a game so sweeping that you really have to wonder if it can be pulled off. ![]() The premise of Microcosmic God Spore is both simple and complex: life from start to finish. Genesis in 5 minutes with Creature Creator from Spore ![]()
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